Bio142 Quiz 5 key

Bio142 Quiz 5 key - Bio142 Lab Spring 2012 Quiz 5 Wednesday...

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Bio142 Lab Spring 2012 Name: Quiz 5 Wednesday 110 Date: 1. (3pts) 1) Define (i.e.what do they stand for) VNTR and STR 2) Describe briefly the difference between them and, 3) how they are used for genetic profiling. VNTR: Variable number of tandem repeats; larger (20-50 base pairs, 50-100s of repeats), uses RFLP Restriction fragment length polymorphism, Restriction Enzyme/southern blot/probes. STR –short tandem repeats shorter (2-4 base pairs, 5-15 repeats), uses PCR. For part 3 of this question I took most anything, because it was poorly worded, but you should understand that VNTRS are analyzed by Southern blot (to big to use PCR) and STRs are analyzed by PCR, you could have either size or detection method as a difference between them 2. Which pipette would you use to measure 0.03ml? (0.5pt) (a) P10, (b) P20, (c) P200 (d) P1000 0.03 X1000= 30 1ml = 1000uL 3. (0.5 point each) What volume is indicated by the following pipettman include UNITS: P20 P200 P1000 5.4uL ______ _ 54uL ______ __ 540uL
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Bio142 Quiz 5 key - Bio142 Lab Spring 2012 Quiz 5 Wednesday...

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