Bio 142 - Week 4 slides - white sp12

Bio 142 - Week 4 slides - white sp12 - L. McLane, K....

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L. McLane, K. McClinic, T. Oliver and A. Escobar
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L. McLane, K. McClinic, T. Oliver and A. Escobar DNA - + DNA moves towards positive charge , WHY? Larger Fragments (more repeats) Smaller Fragments (less repeats)
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L. McLane, K. McClinic, T. Oliver and A. Escobar #3 #16 #8 Unknown DNA Marker (known sizes)
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Before loading your gel, write down order in which your group will load your samples!! Load DNA marker (“ladder”) in left-hand lane followed by your PCR products L. McLane, K. McClinic, T. Oliver and A. Escobar
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L. McLane, K. McClinic, T. Oliver and A. Escobar DNA - (BLACK) + (RED) RUN TO THE RED!
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Connect electrodes Run 45-60 minutes at 120V Take pictures using UV box Analysis L. McLane, K. McClinic, T. Oliver and A. Escobar
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 Need to confirm the presence of the PCR  product BEFORE RFLP. L. McLane, K. McClinic, T. Oliver and A. Escobar
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Breaks the DNA sample into smaller pieces (hence, “fragment”), and then the pieces can be separated by size on a gel. Restriction enzymes are known to cut strands of DNA at specific regions and can be added to a DNA sample which has been subjected to the Polymerase Chain Reaction. http://www.scq.ubc.ca/wp-content/endonuclease2.gif Wheeler and Waikel R estriction F ragment L ength P olymorphism (RFLP)
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The patterns of the bands that result from restriction digests in exons 6 and 7 will determine blood type. The digests will produce fragments of different sizes; the presence of these different bands will determine your blood type!
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This note was uploaded on 04/02/2012 for the course BIO 142L taught by Professor Conniearthur during the Spring '12 term at Emory.

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Bio 142 - Week 4 slides - white sp12 - L. McLane, K....

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