311C DNA_Worksheet - How many of these sites are typically...

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NAME ________________________________ Bio 311C Worksheet : 1. List the three components of a DNA nucleotide. What is a dNTP? 2. What are the base-pairing rules in a DNA double helix (which bases pair together across double helix)? How many hydrogen bonds form between each base-pair? 3. How would the results of Avery, McCarty and MacLeod have differed if protein was the genetic material? Hershey and Chase? 4. Briefly explain the semi-conservative model of DNA replication. 5. Draw the results expected from the famous Meselson and Stahl experiment if the bacterial cells were allowed to replicate three times (3 rounds of DNA replication) in the medium containing 14 N assuming (a) Semi-conservative DNA replication. (b) Conservative DNA replication. 6. What is the site of DNA replication initiation called?
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Unformatted text preview: How many of these sites are typically found in a prokaryotic chromosome? How many in a eukaryotic chromosome? Why a difference? 7. What is the specific reaction catalyzed by DNA polymerase? 8. To which end of the leading strand does DNA polymerase add dNTPs? To which end of the lagging strand? 9. What is an Okazaki fragment? Where do we find Okazaki fragments? 10. What is the function of primase? Why does DNA replication require this enzyme? 11. Many other enzymes and proteins are involved in DNA replication briefly describe the function of each of the following in replication: (a) Helicase: (b) Topoisomerase (c) Single-stranded binding protein (d) DNA ligase 12. What is the importance of the 3-to-5 exonuclease activity of DNA Pol? 13. What is a telomere? What is the function of a telomere?...
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311C DNA_Worksheet - How many of these sites are typically...

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