Ch 16 Bio - Chapter 16 Recombinant DNA and Biotechnology...

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Chapt er 16, page 1 Chapter 16 Recombinant DNA and Biotechnology ---A-G-G-C-T-C-G- G-A-A-T-T-C -T-T-A-C-C-C-G-A--- 5 ' 3' 5' 3' | | | | | | | | | | | | | | | | | | | | | ---A-G-G-C-T-C-G- G A-A-T-T-C -T-T-A-C-C-C-G-A--- 5' 3 ' 5' 3' | | | | | | | | ---T-C-G-G-G-T-A-A- G-A-A-T-T-C -C-G-A-G-C-C-T--- A-A-T-T-C -C-G-A-G-C-C-T--- ---T-C-G-G-G-T-A-A- G | | | | | | | | | 3' 5' 5' 3' 5' A-A-T-T-C -T---- ----A- G | | 3' ----T-C-G- G-A-A-T-T-C -T---- 3' 5' | | | | | | | | | | ---A- G-A-A-T-T-C -C-G-A--- 5' EcoR1: 5' overhang 5' G -T---- ----A- C-T-G-C-A | | 3' ----T-C-G- C-T-G-C-A-G -T---- 3' 5' | | | | | | | | | | 5' PstI: 3' overhang ---A- C-T-G-C-A-G -C-G-A---
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Chapt er 16, page 2
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Chapt er 16, page 4 sequencing
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Chapt er 16, page 8 RNAi use synt hesized dsRNA or make cells express a specific dsRNA
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Chapt er 16, page 9 Individual: 1 2 3 4 5 6
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Chapt er 16, page 10
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Chapt er 16, page 11 Glyphosat e (Round-up) plant EPSP synt hase Aromat ic amino acids bact erial EPSP synt hase Precursors
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Chapt er 16, page 12 Chapter 16 Key Words Clone Electrophoresis DNA ligase Expression vector Gene library Recombinant DNA technology Reporter gene Restriction site Restriction endonuclease Sticky ends Transfection Transgenic organism Vector Yeast artificial chromosome (YAC) Antisense nucleic acid Biotechnology Complementary DNA (cDNA) DNA chip
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Chapt er 16, page 13 Chapt er 16 Highlights: I. Cleaving and Rejoining DNA A. Restriction enzymes cleave DNA at specific sequences.
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