2011_Questions_Week_1_Answers (1)

2011_Questions_Week_1_Answers (1) - You would like to...

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You would like to perform experiments that explore the role of the genes Sall1 and Pitx1 in zebrafish ( Danio rerio ) development. 1. You decide to assay Sall1 expression by in situ hybridization. Unfortunately, you do not have a construct in your laboratory for the zebrafish Sall1 gene. However, a neighboring laboratory has an probe for the mouse Sall1 gene. Do you have enough information to determine if the probe against the mouse gene would work for in situ hybridization experiments in zebrafish? If so, describe why you think they would work. If not, what additional information do you need? -It is highly unlikely to work. However, you do not have enough information to say conclusively because you do not know how closely the fish and mouse genes resemble each other. If the probes extremely high conservation on the nucleic acid level (virtually identical), they experiment would work. On the other hand if the nucleic acid sequences are substantially different, the probe would not hybridize to the fish gene. 2. There are no probes available for Pitx1 so you need to make your own. You generate a plasmid that contains the entire cDNA sequence for the zebrafish Pitx1 gene. The first
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2011_Questions_Week_1_Answers (1) - You would like to...

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