2011_Questions_Week_4_Monday_Answers

2011_Questions_Week_4_Monday_Answers - 1. What happens in...

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1. What happens in C. elegans Pie-1 mutants? ---They no longer make germ cells (P-lineage); instead the P2 descendants become EMS-type cells. 2. In C. elegans, are P-cells an example of autonomous or conditional specification? Why? ---Autonomous; the critical cytoplasmic determinants (primarily P-granules and Pie1) localized to the P-cells, which give rise to the germ cells. They do not depend upon any inductive interactions with other cells to make this choice. 3. You know that Pie-1 protein is localized to and critical for P-cell specification. You want to determine HOW Pie-1 is localized to the P-cells during early cleavages. Describe an experiment that would tell you if Pie-1 localization occurs at the mRNA or protein level. ---Do in situ hybridization to detect Pie-1 mRNA - do you see mRNA in all the blastomores or only in the P-blastmore? If in all blastomores (which it in fact is in at least through the 4 cell stage), it suggests that the mRNA is not regulated but rather that regulation takes place at the protein level. If it the mRNA was localized to the P
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2011_Questions_Week_4_Monday_Answers - 1. What happens in...

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