2011_Questions_Week_4_Wednesday_Answers (1)

2011_Questions_Week_4_Wednesday_Answers (1) - 1. Generate...

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1. Generate germline mosaic adult flies by grafting Cactus mutant pole cells onto wild- type embryos, whose own pole cells have been removed. What would be the phenotype, if any, for the resulting embryos derived from a cross of these mosaic females with wild-type males? ---The resulting germline chimera would have wild-type follicle cells with a Cactus mutant oocytes. Since Cactus a maternal effect protein that is required within the embryo to sequester Dorsal outside the nucleus unless degraded by phosphorylation via a ventralizing signal from activated Toll-receptor, all the embryos will have Dorsal expressed in the nucleus in all cells in a non-graded fashion. This should generate highly ventralized embryos. 2. Generate germline mosaic adult flies by grafting wild-type pole cells onto Cactus mutant embryos, whose own pole cells have been removed. What would be the phenotype, if any, for the resulting embryos derived from a cross of these mosaic females with wild-type males? ---This generates the reciprocal cross of #1: Female flies will have wild-type oocytes and Cactus mutant follicles cells. But Cactus isn't required in follicle cells so all embryos
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2011_Questions_Week_4_Wednesday_Answers (1) - 1. Generate...

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