Lecture 3 DNA replication DNAPs

Lecture 3 DNA replication DNAPs - DNA replication pt2: DNA...

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DNA replication pt2: DNA Polymerases. ..and other enzymes 1 Wednesday, August 31, 2011
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Topic Change to Syllabus Current Version: L3 DNA replication pt 2 enzymes/DNAPs L4 DNA replication pt 3 Nuclear and mtDNA L5 DNA recombination Modifcation : L3 DNA replication pt 2 enzymes/DNAPs L4 DNA replication pt 3 DNAP III/ mtDNA L5 DNA replication pt 4 linear c’somes/telomerase 2 Wednesday, August 31, 2011
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Last Time How to critically analyze scientifc papers General Features oF DNA replication: DNA is replicated semi-conservatively The origin oF replication is non-random (15N/ 32P experiment) Replication is bi-directional (‘mustache’ experiment) 3 Wednesday, August 31, 2011
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DNA replication pt 2 Review the general function of replication proteins Explore the structure and function of prokaryotic DNAPs 4 Wednesday, August 31, 2011
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Learning Outcomes 1. Describe experimental evidence for supporting the Fnding that DnaB is a helicase and that its activity is modulated by other replisome proteins. 2. Apply the experimental process of #1 to a new problem or question. 3. Identify the roles of Replisome enzymes (review), including the three DNAPs found in prokaryotes 4. Describe the “trombone model” of replication and explain how the leading and lagging strands are replicated at the same time by the same enzyme. By the end of this lecture, you should be able to: 5 Wednesday, August 31, 2011
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Basic mechanics of replication DNA polymerases synthesize DNA Replication produces leading and lagging strands DNA ligases join DNA strands together 6 Wednesday, August 31, 2011
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5' 3' 3' 5' 5' 5' DNA Polymerase III acts here DNA Ligase joins fragments together. ssDNA binding protein ( SSB ) Helicase (dnaB) Primase 3' Gyrase S p i n n i n g a t 1 0 ,0 0 0 r p m Primosome Replisome 7 Wednesday, August 31, 2011
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Functions of replication proteins in E. coli photo courtesy: wikipedia 1. DNAP 2. Primase 3. Ligase 4. Helicase 5. Gyrase 6. SSB (s
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Lecture 3 DNA replication DNAPs - DNA replication pt2: DNA...

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