Lecture 4 DNA replication pt 3

Lecture 4 DNA replication pt 3 - DNA replication Part 3:...

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DNA replication Part 3: The beta clamp The phases of replication 1 Friday, September 2, 2011
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Last Time A review of proteins that make up the Replisome Experiment : dnaB is a functional helicase and dnaB’s function is enhanced by the presence of other Replisome proteins. E.coli has 3 polymerases: DNAPI, DNAPII, DNAPIII. DNAPIII, the main replication enzyme, has 10 subunits Core subunits are important for polymerization, gamma complex mediates attachment to DNA via beta clamp 2 Friday, September 2, 2011
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Learning Outcomes 1. Describe experimental evidence supporting the fnding that the β -Clamp interacts with DNA like a “ring on a string”. 2. Apply the experimental process oF #1 to a new problem or question. 3. Describe the process oF replication initiation and termination (in proks). 4. Describe how the origin oF replication was identifed ( experiment ) and be able to apply this technique to a new problem. By the end of this lecture, you should be able to: 3 Friday, September 2, 2011
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DNA replication Pt3 The gamma complex of DNAPIII and the role of the beta clamp Three phases of prokaryotic replication initiation elongation termination 4 Friday, September 2, 2011
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As a general rule, polymerases (or core polymerase subunits) do not bind DNA very well! 5 Friday, September 2, 2011
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Subunits of E. Coli Polymerase III holoenzyme Fig 21.17 Table 20.2 6 Friday, September 2, 2011
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. “Clamps” α subunit onto DNA Fig. 21.13 The β - Clamp is part of the DNAPIII holoenzyme 7 Friday, September 2, 2011
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Hypothesis: The beta-clamp interacts with the DNA like a ‘ring on string’ We frst need to fgure out how to determine when clamp is a) ON the DNA and b) OFF the DNA 8 Friday, September 2, 2011
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Experimental Design B-clamp 9 Friday, September 2, 2011
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This note was uploaded on 03/29/2012 for the course BIO 359K taught by Professor Mcclelland during the Spring '08 term at University of Texas at Austin.

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Lecture 4 DNA replication pt 3 - DNA replication Part 3:...

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