EXAM 1 In Class Review Discussion Questions

EXAM 1 In Class Review Discussion Questions - From Lecture...

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From Lecture 2: DNA Replication pt1 Wednesday, September 14, 2011
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Learning Outcomes 1.Defne each section oF a scientifc article 2.Put into practice the strategy For analyzing scientifc articles 3.Describe the experimental evidence supporting the fnding that a.replication begins as the same site each time b.replication is bi-directional 4.Use the experimental designs presented in the application or interpretation oF a new problem By the end of this lecture, you should be able to: Wednesday, September 14, 2011
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Self Check (individual) 1. What’s the difference between a nucleoside and a nucleotide? 2. Name 3 forces holding together DNA strands (to form a double helix). 3. Why would proteins binding speciFc sequences of DNA more likely bind the major groove? Wednesday, September 14, 2011
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GROUP WORK 4. What are the two hypotheses of this experiment? 5. Why did the DNA need to be sheared before quantiFcation of the radioactivity? 6. In the results, 50% of the P32 was associated with N15. Why would it be impossible to obtain more than 50% radioactivity associated with N15? 7. If none of the radioactivity was associated with N15, which hypothesis would the data support and why? Wednesday, September 14, 2011
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1. Draw out what we mean by the statement “DNAP attaches nucleotides to the 3’ end of the growing strand.” Additional Questions 2. How is the Introduction section of a paper different from the Discussion? 3. Using the “mustache” experimental design, what pattern would you expect to see if replication was unidirectional? Wednesday, September 14, 2011
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From Lecture 3: DNA Replication pt2 Wednesday, September 14, 2011
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Learning Outcomes 1. Describe experimental evidence for supporting the Fnding that DnaB is a helicase and that its activity is modulated by other replisome proteins. 2. Apply the experimental process of #1 to a new problem or question. 3. Identify the roles of Replisome enzymes (review), including the three DNAPs found in prokaryotes 4. Describe the “trombone model” of replication and explain how the leading and lagging strands are replicated at the same time by the same enzyme. By the end of this lecture, you should be able to: Wednesday, September 14, 2011
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(Individual) 1. You continuously add 15 N to a fask oF bacteria and allowed them undergo one cell division. You collect the DNA From the bacteria and separate it on a CsCl gradient. What percentage oF 15 N- 14 N and 14 N- 14 N DNA do you expect? 2. In the ‘mustache’ experiment, what pattern would you expect to see on the X-ray ±lm iF the weaker radioactivity was added ±rst? Wednesday, September 14, 2011
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EXAM 1 In Class Review Discussion Questions - From Lecture...

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