Lecture 8 Prok Trxn 1 Overview Enzymology

Lecture 8 Prok Trxn 1 Overview Enzymology - P ROKARYOTIC T...

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PROKARYOTIC TRANSCRIPTION: MECHANISM AND ENZYMOLOGY http://www.rockefeller.edu/labheads/darst/images/ holo.htm 1 Wednesday, September 21, 2011
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TRANSCRIPTION QUESTIONS How does RNAP bind to DNA? How is dsDNA opened and kept open? How does RNAP move along template strand? How does RNAP know when to stop? Which genes get transcribed? How does transcription machinery find the ‘right‘ gene? How do transcription proteins attach to DNA? 2 Wednesday, September 21, 2011
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MECHANISM AND ENZYMOLOGY Overview Enzymology RNA polymerase subunits and function Transcription in Three Steps Initiation Elongation Termination - next time 3 Wednesday, September 21, 2011
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Learning Outcomes 1. List the important subunits of prok RNAP and know their basic function in transcription 2. Describe the relationship between afFnity of RNAP to DNA and gene expression level, and ways in which this can be modulated. 3. Discuss the signiFcance of the sigma subunit in identifying targets for transcription. 4. Describe the phases of transcription and the important features of each phase. By the end of this lecture, you should be able to: 4 Wednesday, September 21, 2011
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E. COLI RNAP Subunits Two large beta subunits: β (150kD) and β ’ (160kD) Two copies of alpha α (40kD) Sigma σ (70kD) Omega ω (10kD) - no role in active enzyme, aids in formation and stability of enzyme by binding B’ 5 Wednesday, September 21, 2011
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Core subunits α , β , β Holoenzyme : α , β , β and σ σ is the specificity factor Form fits function! 6
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This note was uploaded on 03/29/2012 for the course BIO 359K taught by Professor Mcclelland during the Spring '08 term at University of Texas at Austin.

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Lecture 8 Prok Trxn 1 Overview Enzymology - P ROKARYOTIC T...

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