Exam 2 review and Discussion Questions

Exam 2 review and Discussion Questions - Exam 2 Learning...

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Exam 2 Learning Outcomes, In Class Discussion and End of Lecture Questions 1 Monday, October 17, 2011
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Lecture 8 2 Monday, October 17, 2011
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Learning Outcomes 1. List the important subunits of prok RNAP and know their basic function in transcription 2. Describe the relationship between afFnity of RNAP to DNA and gene expression level, and ways in which this can be modulated. 3. Discuss the signiFcance of the sigma subunit in identifying targets for transcription. 4. Describe the phases of transcription and the important features of each phase. By the end of this lecture, you should be able to: 3 Monday, October 17, 2011
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Self Check - What do you remember about transcription? 1. What are transcription factors? 2. For a given cell, is the entire genome transcribed? Why/ why not? 3. Is a primer required in transcription, as in replication? 4. How does RNAP ±nd the ‘right’ gene to transcribe? 5. In what part of the cell does transcription occur? 4 Monday, October 17, 2011
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Group Work 4. Since sigma is the “specifcity Factor”, would you anticipate that it is binding to a specifc sequence oF DNA? Why/Why not? 3. What do you think would happen to transcription iF RNAP could not bind the sigma Factor? 1. What does it mean when we say a sequence is ‘conserved’? Why is this signifcant? 2. What can we deduce when we see that the spacing between two sequences is conserved? 5 Monday, October 17, 2011
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Group Work 7. What do you notice about this fgure? How can you relate how sigma binds DNA to what you know about prok promoter sequences? 5. What’s a protein domain? 6. What Functions do you anticipate a sigma subunit would have? -10 -35 6 Monday, October 17, 2011
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1. The specificity of DNA binding by the E. coli RNA polymerase is provided mainly by which subunit? 2. How can you manipulate a promoter to strengthen or weaken transcription of a given gene? 3. How does RNAP correct errors made while transcribing DNA? 4. What functional domains of sigma are required for binding the -10 and -35 box? 5. Why would transcriptional control of a single sigma factor gene affect entire collections of genes? Additional Questions 7 Monday, October 17, 2011
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Lecture 9 8 Monday, October 17, 2011
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1.Describe the phases of transcription and the important features of each phase. (For example: Distinguish between the open and closed complex in initiation, discuss important factors that promote RNAP’s movement into elongation and distinguish between Rho-dependent and independent termination.) 2.Describe how the lac operon is regulated, including all important DNA and protein components. 3.Discuss how the LacI repressor inhibits lac operon expression, including the two hypothesis and the data that support these hypotheses. [may fnish this in the next lecture] . By the end of this lecture, you should be able to:
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Exam 2 review and Discussion Questions - Exam 2 Learning...

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