BIO 1140 DGD March 24 - activator protein CAP active CAP(or...

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BIO 1140 DGD March 28, 2011
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Bacterial gene regulation: the lac operon Genes involved in lactose metabolism: lacZ, lacY, lacA  all next to each other  expressed only when an inducer (ie lactose) is present Belong to an operon  group of genes with related functions that are clustered together with DNA sequences that allow the genes to be turned on and off simultaneously. lacI  encodes for the repressor
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lac operon
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Positive vs negative control of transcription Negative control  repressor in the effector – free state is active for catabolic pathways  results in inhibiting gene transcription Positive control  ensures that other carbon sources are used only when glucose is not available  glucose present ≠ cAMP  cAMP is needed to bind to cAMP receptor protein (CRP or cAMP activator protein CAP)  active CAP (or
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Unformatted text preview: activator protein CAP) active CAP (or Eukaryotic gene regulation • Regulated at the levels of the genome, transcription, RNA processing and export from the nucleus to cytoplasm, translation and postranslational events • Different sets of genes are transcribed in different cell types differentiation • Proximal control elements located upstream of the core promoter common sequences: CAAT box, GC box, octamer bind regulatory transcription factors Steroid hormone regulation Practice questions 1.) what would be the phenotype of a lacI- E. coli (i.e. lac repressor mutant) strain growing on minimal medium with lactose as the only carbon source? 2.) What would be the phenotype of an E. coli strain that had a mutation in its CAP gene (i.e., Cap-)?...
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