Clickers_10-12

Clickers_10-12 - + + protease + + microsomes + + + + 35S...

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Unformatted text preview: + + protease + + microsomes + + + + 35S meth reticulocyte lysate + + + + mRNA 2 + + + + mRNA 1 55kDa 43kDa SDS-PAGE Q. Which protein would you suspect is normally secreted? A. Protein 1 (black) B. Protein 2 (red) C. Both. D. Neither one. Conclusions : Lane1=translation occurs Lane 2= cleavage of protein 1 in presence of microsomes suggests translocation and signal peptidase activity. No change in protein 2. Lane 3= protease degrades both proteins Lane 4=protease shows that protein 2 is not protected, therefore not translocated and protein 1 is protected, therefore translocated. Not all proteins showed same pattern, specific for secreted proteins 55kDa 50kDa 50kDa protease Detergent Signal peptidase Protein 2 Protein 1 Clicker Question mRNA 3 + + 35S meth reticulocyte lysate + + microsomes + SDS-PAGE Q. Hypothesize why is the band in lane 1 smaller than the band in lane 2? A. The protein in lane 2 is unfolded B. The band in lane 1 has had its signal peptide cleaved C. The protein encoded for by mRNA 3 is a transmembrane protein. D. The band in lane 2 has a signal peptide E. Sugars were added to the protein in lane 2 How would you test this hypothesis? Lumenal reactions: mRNA 3 + + + + + + + + 35S meth reticulocyte lysate + + + + + + + + microsomes + + + + + + protease + + + + tunicamycin...
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This note was uploaded on 03/31/2012 for the course BIS 104 taught by Professor Scholey during the Winter '08 term at UC Davis.

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Clickers_10-12 - + + protease + + microsomes + + + + 35S...

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