Clickers_7-9

Clickers_7-9 - C. In the cytoplasm D. In the ER lumen E....

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Using Yeast genetics to study the secretory pathway Clicker question A sec12 mutant yeast cell has a lack of vesicles and an larger ER as compared to wild-type. A sec17 mutant has an excess of vesicles and a small ER as compared to wild-type. What would you predict a sec12, sec17 double mutant would look like? A. Extra vesicles and a small ER B. Too few vesicles and a large ER C. Normal number vesicles and a large ER D. Normal number of vesicles and a normal ER E. Normal number of vesicles and a small ER
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Clicker question Q. You have isolated a yeast strain with a mutation in the GTP binding domain of SRP. This mutant SRP can not bind a nucleotide. Where would you predict to find a protein that is normally secreted? A. Never made B. Partially made, but still attached to ribosomes
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Unformatted text preview: C. In the cytoplasm D. In the ER lumen E. Secreted outside the cell Q. SRP remains stably bound to the SRP receptor at the ER membrane when _________? A. SRP receptor is associated with the translocon B.When the translocon acts as a GAP on the SRP receptor C. When SRP acts as a GAP on the SRP receptor D. When SRP and SRP receptor are bound to GTP E.When SRP hydrolyzes GTP Clicker Question + microsomes + + 35S meth reticulocyte lysate + + mRNA 1 55kDa SDS-PAGE Q. Why is the band in lane 2 smaller than the band in lane 1? A. The protein in lane 1 is unfolded B. The band in lane 1 has a signal peptide C. The protein encoded for by mRNA 1 is a transmembrane protein. D. The band in lane 2 has a signal peptide E. Sugars were added to the protein in lane 1...
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This note was uploaded on 03/31/2012 for the course BIS 104 taught by Professor Scholey during the Winter '08 term at UC Davis.

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Clickers_7-9 - C. In the cytoplasm D. In the ER lumen E....

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