Handouts_14-18

Handouts_14-18 - + N Golgi: carried by dynein, -end of...

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N centrosome microtubules ER: pulled out to plus end of Mt ` s Golgi: carried by dynein, -end of microtubules - + COPI-coated vesicles: KDEL receptor carrying vesicles - + golgi ER +/- are not charges; conformation Kinesin based transport + end directed movements
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Filament Dynamics Two ways to generate force Motor proteins Filament dynamics ---Time course of polymerization in a test tube ---Rate constants (depend on temp, salt) k on =rate constant for the addition of one subunit to a polymer off =rate constant for the loss of one subunit from a polymer is dependent on the concentration of free subunits ( C ). So does not depend on Critical Concentration is when the concentration of free subunits drops until the on and off rates are the same: = c = off / - + C c =0. 8 μ Μ C c =0.1 μ M
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High Cc 0.8 uM ADP depolymerization Barbed (+) end Low Cc 0.1 uM ATP growth
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This note was uploaded on 03/31/2012 for the course BIS 104 taught by Professor Scholey during the Winter '08 term at UC Davis.

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Handouts_14-18 - + N Golgi: carried by dynein, -end of...

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