Problem_set_1_key

Problem_set_1_key - BIS104 Winter 2012 Problem Set I Due...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: BIS104 Winter 2012 Problem Set I Due Fri Jan 20 This problem set will not be collected. However, I will know whether or not you did it by your performance on the exam. 1-3. In studying normal cells, you isolate gene R that normally sequesters and turns off transcription factor E. When active, transcription factor E binds to the promoter of gene A, which encodes a protein that accelerates the cell cycle. 1. You isolate a tumor and find that it has a homozygous null mutation in gene R. In the tumor, transcription factor E is now hyperactive and Gene A is rapidly transcribed. You conclude the following: A. Protein R is sufficient for the inactivation of transcription factor E B. Protein R is necessary for the inactivation of transcription factor E C. Protein R is both necessary and sufficient for the inactivation of transcription factor E 2. If you concluded that protein R is sufficient, describe an experiment to test whether protein R is necessary. If you concluded that protein R is necessary, describe an experiment to test whether protein R is sufficient. (You have a known promoter that you know transcription factor E activates). To test if protein R is sufficient to inactivate transcription factor E you first need to set up a system where you can measure the activity of transcription factor E. I would put the promoter of geneA into a bacteria or yeast (or even a test tube) with a reporter. Then add transcription factor E, yeast (or even a test tube) with a reporter....
View Full Document

This note was uploaded on 03/31/2012 for the course BIS 104 taught by Professor Scholey during the Winter '08 term at UC Davis.

Page1 / 4

Problem_set_1_key - BIS104 Winter 2012 Problem Set I Due...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online