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Unformatted text preview: BIS104 Winter 2012 Problem Set I Due Fri Jan 20 This problem set will not be collected. However, I will know whether or not you did it by your performance on the exam. 1-3. In studying normal cells, you isolate gene R that normally sequesters and turns off transcription factor E. When active, transcription factor E binds to the promoter of gene A, which encodes a protein that accelerates the cell cycle. 1. You isolate a tumor and find that it has a homozygous null mutation in gene R. In the tumor, transcription factor E is now hyperactive and Gene A is rapidly transcribed. You conclude the following: A. Protein R is sufficient for the inactivation of transcription factor E B. Protein R is necessary for the inactivation of transcription factor E C. Protein R is both necessary and sufficient for the inactivation of transcription factor E 2. If you concluded that protein R is sufficient, describe an experiment to test whether protein R is necessary. If you concluded that protein R is necessary, describe an experiment to test whether protein R is sufficient. (You have a known promoter that you know transcription factor E activates). To test if protein R is sufficient to inactivate transcription factor E you first need to set up a system where you can measure the activity of transcription factor E. I would put the promoter of geneA into a bacteria or yeast (or even a test tube) with a reporter. Then add transcription factor E, yeast (or even a test tube) with a reporter....
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This note was uploaded on 03/31/2012 for the course BIS 104 taught by Professor Scholey during the Winter '08 term at UC Davis.
- Winter '08