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Problem_set_2_key - BIS104 Winter 2012 Problem Set 2"Due...

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BIS104 Winter 2012 Problem Set 2 “Due” Fri Feb 3 This problem set will not be collected. However, I will know whether or not you did it by your performance on the exam. 1-3. Revisit the paper you read for Problem Set #1, “Global analysis of protein localization in budding yeast” Nature. 2003 Oct 16;425:686-91. A PDF version of the paper is available on the class website. Application: Go the web site that provides images of every GFP fusion from the above study (http://yeastgfp.yeastgenome.org/). Feel free to explore the site as you answer the following questions: 1. Compare the localization patterns of srp14 and sec31. Where do they localize? Srp14: around the nucleus in a pattern that is consistent with the endoplasmic reticulum Sec31: in vesicles on their way to the golgi compartment 2. Although they are both involved in secretion, do their localization patterns suggest that they have different roles. What is the biochemical function of srp14 and sec31 (click on the blue YLR or YDL name on the yeastgfp entry and read the description on the SGD page)? Srp14: part of the SRP that arrests protein translation Sec31: is part of the COPII complex involved in anterograde vesicle transport of
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