Problem_set_4

Problem_set_4 - BIS104 Winter 2012 Problem Set 4 1. You...

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Unformatted text preview: BIS104 Winter 2012 Problem Set 4 1. You briefly (about 15 minutes) label tissue culture cells with radioactive H 3 thymidine. You then look at time points at later times. At each time point, you collect the cells in mitosis and quantitate the percentage of cells that are labeled with radioactive thymidine (see the graph). What is the length of G2 in these cells? 3 6 9 12 15 18 21 24 27 30 33 35 Time in hours after H 3 thymidine pulse %o fM- phasecellsthatare labeledwithH 3 Thymidine 2-5. You have microinjected a newt lung cell with rhodamine-labeled tubulin, c aged- fluorescein labeled tubulin and the fluorescent DNA stain, DAPI. You wait until the cell enters metaphase and you uncage a bar of caged-fluorescein tubulin in the spindle. The figures below show the fluorecence intensity of uncaged fluorescein on the y axis and the distance from the left spindle pole (centrosome) on the x axis. The position of one sister chromatid that is attached to the left pole by a kinetochore fiber is also shown. 2. What is the rate of poleward flux in m min-1 ?...
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Problem_set_4 - BIS104 Winter 2012 Problem Set 4 1. You...

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