ProblemSet06 - BC367, Fall 2011 Problem set #6 Due: Friday,...

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Unformatted text preview: BC367, Fall 2011 Problem set #6 Due: Friday, October 21 (Each problem must be completed on a separate page. All short answer questions must be typed. All math problems must show work.) 1. If an enzyme enhances the rate of a particular enzymatic reaction by 5.3 x 1011 fold, what is the difference in the activation energies of the catalyzed and uncatalyzed reactions? 2. Use the following enzyme‐catalyzed reaction data set to calculate the maximum velocity and the Michaelis constant of the enzyme using the Lineweaver‐Burk equation. [S] (mM) Vo (uM/min) 1 2 4 6 8 10 12 14 16 18 12 29 40 55 67 76 82 85 88 91 20 96 3. Calculate the same values using least‐squares curve fitting. (Print out and attach your spreadsheet) 4. Derive a velocity equation for a random bisubstrate enzyme. ...
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This note was uploaded on 03/31/2012 for the course CHEM 360 taught by Professor Millard during the Spring '12 term at Colby.

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