ProblemSet04

ProblemSet04 - the following types of protein...

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BC367, Fall 2011 Problem set #4 Due: Friday, September 30th (Don’t forget that each question should be answered on a separate sheet of paper. Also, please type your narrative answers.) 1. In addition to the more typically used modes of liquid chromatography (ion exchange, size exclusion, affinity, etc.) some scientists have found that a chromatographic stationary phase that includes the mineral hydroxyapatite (HA) can be useful as separating some proteins. Do some brief research on HA chromatography and describe what it is and how it could be used. (Your answer should be cited.) 2. You are trying to purify a protein that can be found in homogenized bacterial culture. You know your protein to be a relatively large, globular enzyme that is especially rich in histidine residues. Describe how you could use each of
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Unformatted text preview: the following types of protein chromatography: ion exchange, size exclusion, and affinity. Make sure to be very specific in the considerations and conditions youd need for each. 3. You are trying to measure the concentration of a purified protein that has 5 aromatic residues (all Trp) using ultraviolet absorbance spectroscopy, but you dont know the actual extinction coefficient. Why might the actual extinction coefficient be different than that of tryptophan itself? Propose an experiment to measure and calculate the actual extinction coefficient. 4. Suggest three reasons why a single protein would resolve as two distinct bands on an SDSpolyacrylamide electrophoretic gel....
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