Exam3_Review

Exam3_Review - spectrometry. 6. Whats the difference...

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My office hours prior to the exam are: Friday, Nov 11, 3-4 pm Monday, Nov 14, 3-5 pm 0128 Bioscience Research Building carenc@umd.edu Review for Exam 3 Thursday Nov 10, 2011 1
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Review questions 1. Suppose you have an Arabidopsis gene and you want to: a) find the mutant phenotype for the gene b) examine the gene’s expression within certain cell types c) identify proteins that interact with the protein encoded by the gene What experiments could you do for each of the above? 3. Describe an experiment you could do with a northern blot. 5. Describe an experiment you could do with a microarray. 2
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4. Describe an experiment you could do with RNA-Seq. 5. Describe an experiment you could do with mass
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Unformatted text preview: spectrometry. 6. Whats the difference between a transcriptional reporter and a translational reporter, and will the patterns be the same? 7. Describe an experiment you could do with a collection of RNAi constructs for every gene in C. elegans . 3 8. Why does targeted gene replacement in mice require a marker for negative selection? 9. Why does targeted gene replacement in mouse require ES cells taken from one color of mouse and a blastocyst from a different color of mouse? 10. All of the tools in the above questions are tools of a) reverse genetics b) functional genomics c) high throughput analysis d) molecular genetics 4...
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This note was uploaded on 04/03/2012 for the course BSCI 410 taught by Professor Staff during the Spring '08 term at Maryland.

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Exam3_Review - spectrometry. 6. Whats the difference...

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