Lecture3 mutant analysis

Lecture3 mutant analysis - Lecture 3: Mutant isolation and...

Info iconThis preview shows pages 1–12. Sign up to view the full content.

View Full Document Right Arrow Icon
Lecture 3: Mutant isolation and Characterization Mutagenesis (mutant isolation) Mutant analysis Recessive vs dominant Complementation tests Read: 224-226 Fig. 7.17, 18, 23a 1
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Mutant Isolation 2
Background image of page 2
Screen: Visual: flower morphology, pigment color,  Examples of transposon induced mutations in eye color and kernel color Biochemical pathway mutant (arg -  in Neurospora; trp1 -  in plant) Reporter gene expression (luc bioluminescent protein from firefly) Selection:  trp pathway: 5-methylanthranilate ---> 5’methyltrp (toxic) ADH (alcohol dehydrogenase): Allyl alcohol---> acrolein aldehyde (toxic) C-Fern project Lethal mutations:  (such as house keeping genes) -maintain as heterozygote -weak hypomorph -conditional such as temperature-sensitive mutants 3 Mutagenesis (forward genetics)
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
WT  Arabidopsis  flower Arabidopsis floral mutant 4
Background image of page 4
5 sex-determination mutant in maize      ( tsl2 =tassel seed 2 )
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 6
7 Fig. 13.28 Transposons can insert into different regions of a gene
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Fig. 7.17
Background image of page 8
9 Auxotroph and Prototroph Complete Medium v.s Minimum Medium
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
10 Fig. 7.23a
Background image of page 10
Tryptophan biosynthetic pathway Chorismate Anthranilate  ( blue fluorescent under UV ) trp4 (AS) trp1(PAT) Phosphoribosyl (PR) anthranilate PAI 
Background image of page 11

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 12
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/03/2012 for the course BSCI 410 taught by Professor Staff during the Spring '08 term at Maryland.

Page1 / 25

Lecture3 mutant analysis - Lecture 3: Mutant isolation and...

This preview shows document pages 1 - 12. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online