Lecture5 molecular techniques

Lecture5 molecular techniques - Lecture 5 Molecular...

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1 Lecture 5 Molecular Techniques 1. Restriction enzymes and restriction digestion 2. Electrophoresis 3. Southern and northern 4. Molecular cloning (recombinant DNA) Inserts and vectors Restriction enzymes and ligase 5. Libraries and screening 6. PCR Fig. 9.2, 9.3, 9.4, 9.5, 9.6, 9.7, 9.11, 9.12 Table 9.1 Read p290-302, 306-313 Anno unc e m e nt: Re vie w this  Thu S e pt. 22 (HW ke y po s te d) O ffic e  ho ur o n Mo nda y S e p. 26/ 1:30-2:30 Exa m  #1 o n Tu S e p. 27 S tudy g uide  will be  po s te d to day
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2 Fig. 9. .2 Restriction enzymes:   The beginning of recombinant DNA technology / Molecular Cloning
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3 3 Restriction enzymes were isolated  from bacteria.   Why do bacteria have them?
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Restriction enzymes (endonucleases) recognize palindromic  sequences of different lengths 4 base cutter (GTAC) 6 base cutter (GAATTC) Which enzyme would cut more frequently? (assuming equal ratio of bases and equal probability that a base is present)
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This note was uploaded on 04/03/2012 for the course BSCI 410 taught by Professor Staff during the Spring '08 term at Maryland.

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Lecture5 molecular techniques - Lecture 5 Molecular...

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