Lecture16 C. elegans

Lecture16 C. elegans - Lecture 16 Model organisms C elegans...

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Lecture 16: Model organisms: C. elegans Some facts about C. elegans 3 days life cycle Invariant cell lineage 959 cells in hermaphrodite 1031 cells in male Small genome 97 MB 19,000 genes (1 gene per 5 kb) Two sexes: hermaphrodite and male Six pairs of chromosomes Read: reference C (uploaded in ELMS under course materials)
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2 Fig. C.1
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3 XX: hermaphrodite XO: male Sex of C. elegans
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4 Fig. C.5b Life cycle
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5 DNA transformation Inject DNA into distal syncytial gonad of hermaphrodites Irradiation promotes integration of transgenes into genome Reporter constructs show transgenes Fig. C.8
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6 Fig. C.7b
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7 Fig. C.5a
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8 Fig. C.7a Invariant cell lineages (959 somatic cells in hermaphrodite)
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9 Sydney Brenner John Sulston Bob Horvits 2002 Nobel Prize Winners For establishing C. elegans as the model organism and the discovery of program cell death genes
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TUNEL assay: T dT-mediated d UTP digoxigenin n ick e nd l abeling Apoptosis patterns in developing Xenopus embryos detected via TUNEL 1. Apoptosis is important in development C. elegans plays an important role in the discovery of programmed cell death (apoptosis) genes Terminal deoxynucleotidyl transferase (TdT) is a DNA polymerase distinct from other DNA polymerases in that it can add nucleotides to the 3’ end of gene segments without the use of a template strand. 2. How to assay apoptosis?
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Lecture16 C. elegans - Lecture 16 Model organisms C elegans...

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