preparation for exam_1

preparation for exam_1 - (1 Terminologies and concept...

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(1) Terminologies and concept Minimal medium, complete medium Auxotroph vs. prototroph Silent, nonsense, missense, neutral mutations Recessive and dominant mutations Conditional mutants Hypomorph (reduction of function), hypermorph (gain-of-function), amorph (null), antimorph (dominant-negative), neomorph (gaining new function) Semi-dominant (incomplete dominance, co-dominance), haploid–insufficient, Reporter (Luciferase) Ectopic (in time and in space) Mutant screen vs. mutant selection Homozygous vs heterozygous Alleles Complementation groups Complementation test Intragenic, extragenic suppressors (one example of each) Cloning Basic tools in cloning (ligase, vector, lacZ, restriction enzymes) Library construction and screen (will be taught this Thu) Colony hybridization vs. Southern hybridization Southern vs Northern Restriction enzyme vs. Restriction map Genotype vs. phenotype Reverse transcriptase vs. transposases vs. Taq polymerase Denature DNA, annealing, hybridization Primers
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preparation for exam_1 - (1 Terminologies and concept...

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