Exam 1 - AGRY 320 2010 Exam 1 ver_1 Name:_ Select the...

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AGRY 320 2010 Exam 1 ver_1 Name:______________________________ Select the correct version number for your exam. Choose the best answer for each question. 1. In one strand of DNA the nucleotide sequence is 5'-ATCC-3'. The complementary sequence in the other strand must be: a) 5'-CGTA-3' b) 5'-ATGC-3’ c) 5'-GGAT-3' d) 5'-ATCG-3' e) 5'-TAGG-3' 2. If thymine makes up 15% of the bases in a certain DNA sample, what percentage of the bases must be cytosine? 1
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a) 15% b) 35% c) 30% d) 50% e) Not enough information to tell. 3. The Central Dogma of molecular genetics can be most accurately outlined as follows: a) DNA RNA by transcription, then RNA Protein by translation b) DNA RNA by translation, then RNA Protein by transcription c) DNA RNA by replication, then RNA Protein by translation d) DNA RNA by replication, then RNA Protein by transcription e) DNA Protein; RNA is very unstable and not considered a permanent part of the process. 4. Which of the following does not occur during post-transcriptional processing of RNA in eukaryotes? a) cleavage of the 5’ end of the mRNA b) addition of a poly-A at the 5' end c) removal of exons d) addition of a 7-methyl guanosine cap at the 3' end e) all of the above 5. Given the DNA sequence of the transcribed region of a gene, (5')- ATGTA C AAGGTCGGATGA -(3'), with the arrow and the bold nucleotide indicate the location and direction of transcription, what is the sequence of the synthesized mRNA? a) (5')-ATGTACAAGGTCGGATGA-(3') b) (5')-AUGTACAAGGUCGGAUGA-(3') c) (5')-TACATGTTCCAGCCTACT-(3') d) (5'-CAAGGUCGGAUGA-(3') e) (5'-CAAGGTCGGATGA-(3') 6. Alternative splicing is defined as: a) the presence of striated and smooth muscle in one operon b) the use of different combinations of splice donors and acceptors of the same hnRNA to generate different polypeptides c) the specific insertion of transposons into genomic DNA which causes splicing of different genes d) none of the above e) all of the above 7. How does an intergenic nonsense suppressor mutation prevent stop codon mutants from having a prematurely terminated polypeptide? 2
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a) The mutation turns the stop codon back into a wild-type codon. b) The mutation alters a tRNA so that it reads the stop codon and inserts an amino acid. c) The mutation alters the release factors that would halt synthesis. d) The mutation results in a wobble that allows synthesis to continue. e) The mutation replaces the stop codon with a different stop codon. 8. Human insulin protein can be produced by bacteria using exons from human DNA, demonstrating that: a) the genetic code is a heteropolymer specifying a many amino acids b) bacteria and humans must have interchangeable genetic codes c) avirulent bacteria can be rendered infectious with exogenous DNA d) a new amino acid is transcribed from a nonsense mutation e) translation requires oligomeric proteins homologous to ribosomal tRNA 9. A messenger RNA is 642 nucleotides long, and contains a single exon that codes for a protein
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This note was uploaded on 04/02/2012 for the course AGRY 320 taught by Professor Stewert during the Spring '09 term at Purdue University-West Lafayette.

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Exam 1 - AGRY 320 2010 Exam 1 ver_1 Name:_ Select the...

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