Lecture+2+basic+bacteriology+FST104

Lecture+2+basic+bacteriology+FST104 - FST 104 Prof Maria...

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1/11/2012 1 January 11, 2012 FST 104 Prof Maria Marco Office hours: Mondays 4 – 5:30 pm 3200 RMI South Bokyung Lee Office hours: Thursdays 11 – 12:30 3143 RMI North Fairy Fan Office hours: Wednesdays 1 – 2:30 3143 RMI North
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1/11/2012 2 See notes from previous lecture regarding: Email and Announcements on Smartsite Exams, pop-quizzes, and extra credit Required manual for the class Viruses Replicate inside living cells and do not self-replicate. Contain genetic material (DNA or RNA), protein coat, and lipid envelope (some) Eukaryotes Organelles, True nucleus Protozoa and helminths (parasitic worms) Fungus: Yeasts and molds Prions Proteinaceous infectious particle. Composed of protein in a mis-folded form. No genetic material Bacteria “Prokaryotes” No organelles, No internal membranes **Most important modern food borne pathogens** [ Archaea Newly recognized domain of life. No pathogens known ] Types of infectious agents
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1/11/2012 3 Outline 1.Ways to distinguish between bacterial cells 2.Extracellular components of bacterial cells 3.Bacterial growth Not intended to replace courses on bacterial physiology, diversity, and genetics!!
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1/11/2012 4 Methods to distinguish between bacteria 1. Laboratory culture medium preferences Nutrition Appropriate sources of Carbon and Nitrogen Vitamins and cell building-blocks Bacteria are not easy to tell apart Temperature Oxygen requirements pH Water activity Methods to distinguish between bacteria 1. Laboratory culture medium preferences 2. Colony morphology
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1/11/2012 5 Methods to distinguish between bacteria 1. Laboratory culture medium preferences 2. Colony morphology 3. Cell shape (microscopy) 4. Cell staining (microscopy) Methods to distinguish between bacteria 1. Laboratory culture medium preferences 2. Colony morphology 3. Cell shape (microscopy)
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6 Gram-stain microscopy technique 4. Cell staining (microscopy) Methods to distinguish between bacteria 1. Laboratory culture medium preferences
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Lecture+2+basic+bacteriology+FST104 - FST 104 Prof Maria...

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