Lecture+3+Enterics+and+Ecoli+_color_

Lecture+3+Enterics+and+Ecoli+_color_ - ENTERIC PATHOGENS...

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FST104 January 13, 2012 ENTERIC PATHOGENS Bacterial Growth: An increase in the number of bacterial cells (not an increase in the size of an individual cell) Bacterial Growth Binary cell division leads to the growth of cells in the population. DNA
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Test your knowledge 1. An exponentially growing bacterial culture contains 1000 cells at 2 pm and 1x10 8 cells 6 h later, what is the specific growth rate (k) of that culture? What is the doubling time (g) of that culture (in minutes)? 2. If k = 1.33 h -1 and there are 1x10 5 cells after 4 h of exponential phase growth, how many cells were there originally in the culture? 1. An exponentially growing bacterial culture contains 1000 cells at 2 pm and 1x10 8 cells 6 h later, what is the specific growth rate (k) of that culture? What is the doubling time (g) of that culture (in minutes)? log 10 N 1 – log 10 N 0 = k (t 1 – t 0 ) / 2.303 g (generation time) = ln2 / k g = 0.693 / 1.92 h -1 0.36 h and in minutes: 0.36 h *60 min/h = 22 min log 10 of 1000 = 3 log 10 of 1x10 8 = 8 8 - 3 = k (6 h) / 2.303; 5 = k * 2.6 h; 1.92 h -1 = k
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2. If k = 1.33 h -1 and there are 1x10 5 cells after 4 h of exponential phase growth, how many cells were there originally in the culture? log 10 N 1 – log 10 N 0 = k (t 1 – t 0 ) / 2.303 5 – log 10 N 0 = 1.33 h -1 (4) / 2.303 log 10 N 0 = 2.69 ~500 CELLS enteric – of or within the intestine Enteric microorganisms Microbes which inhabit the intestine Enterobacteriaceae (family) Gram negative (G-) Facultative anaerobes Rod shaped Enterobacteriaceae include intestinal parasites of animals and/or humans Others are pathogens of plants or are harmless
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Lecture+3+Enterics+and+Ecoli+_color_ - ENTERIC PATHOGENS...

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