Lecture+15+Temperature+_part+2_+_color_

Lecture+15+Temperature+_part+2_+_color_ - Biological...

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Biological effects of temperature February 15, 2012 FST104 Approximate grade ( including quiz scores ) …only intended as an indicator! 80 or above: A 65 - 79: B 50 - 64: C 49 or less: D Midterm 1 Midterm 2: Material from Feb 13 th Final: Cumulative Collect scantrons and exams during office hours or Friday after class
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Temperature and growth T opt: 3-5 ˚C span Growth rate decreases rapidly above T opt Growth rate (K or μ) Increasing Temp, 5 º C intervals MIN MAX OPT 40 ˚C Q 10 = rate at temperature T+ 10 ºC = ~2 rate at temperature T Between T min and T opt Effect of temperature on survival Slower growth rate = better survival Above T opt = no growth and poor survival (except endospores)
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Temperature and microbial structure and physiology Biological basis of temperature limits 1. membrane structure (phospholipid bilayer) 2. proteins T mp melting point (freezing point) T bp boiling point Any given lipid has a characteristic T mp and T bp Lipid viscosity: function of temperature Increasing fluidity
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Temperature tolerance is related to membrane lipid composition Key feature in heat tolerance: degree of fatty acid saturation Cell membrane and temperature High T mp Low T mp Functions of the cell membrane: - Exclusion of toxic compounds - (Active) Transport of nutrients into & out of the cell - Scaffold for cytoplasmic proteins Cell membrane: organized, yet fluid
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Low temperatures Lipids solidify Membrane reactions are inhibited High temperatures Lipids will be fluid and unable to maintain optimal structural
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Lecture+15+Temperature+_part+2_+_color_ - Biological...

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