BIO 325L Lecture 2

BIO 325L Lecture 2 - This Week In Lab. Lab activities: Quiz...

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This Week In Lab…. Lab activities : Quiz Fly lab #2: P-generation crosses Fly lab #3: D. virilis Polytene preparations Sordaria lab #1: Inoculate cultures
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What do you need to turn in? Plagiarism homework – two parts Essay topic selection Writing assignment (WA) #2 Topic description (brief paragraphs) 2
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Anesthetizing flies- using CO2 Too little…flies fly off… & drown in someone’s coffee.  Just right…flies can be sorted, sexed, transferred, offspring counted, etc. before they wake up!  Separating males from females – How to… ?? Last TIME…
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http://flymove.uni-muenster.de/ The two best ways to distinguish male from female are to look at external genitalia and sex- combs. Coloring is not as good because very young flies students will have very little pigment.
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http://flymove.uni-muenster.de/ Close-up of sex-combs on forelegs of males, but not females. They are used by the male for mounting the female during mating.
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to deduce the patterns of inheritance of Drosophila traits. Practice in working with model organisms data compilation and analysis making valid null-hypotheses testing validity of the null-hypotheses using Chi-square test Drosophila mating crosses: Purpose
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Week 1 – Mating Cross (P X P) Week 2 – Discard Breeding Parent Generation Week 3 – Score the F1 Progeny; F1 X F1 Cross Week 4 – Discard Breeding Adults Week 5 – Score F2 Progeny Lab schedule for fly breeding
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Drosophila Notation All mutations are compared with wild-type. Wild-type is represented by a plus symbol. Example: Instead of writing “ AA” , we write +/+ . The slash divides the parental alleles of the gene being studied.
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Notation An allele that is recessive to wild-type is represented by a letter or group of letters that are all lower-case . A female fly received a
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This note was uploaded on 04/02/2012 for the course BIO 325L taught by Professor Franks during the Spring '08 term at University of Texas.

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BIO 325L Lecture 2 - This Week In Lab. Lab activities: Quiz...

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