BIO 325L Lecture 5 - This week in lab 1 Quiz Turn in...

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This week in lab… Quiz Turn in homework Lab activities Delete F1 generation PV92 part 2 – gel and results STR “crime lab” PCR set up Lab practical: Microscope use ( Sordaria slide prep) Note: there will be a lecture given on 3/5 1
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What do you need to turn in? Homework assignment Prelab/homework assignment (Includes PV92 data analysis) 2
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Today in lecture PV92 and Hardy-Weinberg Introduction to statistics 3
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PV92 results Set up, load, and run gel Stain, destain, and determine results “Positive and negative controls” vs. “plus and minus alleles” 4 941 bp 641 bp - +/- +
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Controls Positive controls An experimental set up that should produce a signal Helps detect “false negatives” Negative controls An experimental set up that should NOT produce a signal Helps detect “false positives” If a PCR set up is not supposed to show a band on the gel, is it a positive or negative control? 5
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What Is Needed for PCR? Template (the DNA you want to amplify for the study) Sequence-specific primers flanking the target sequence Nucleotides (dATP, dCTP, dGTP, dTTP) Magnesium ions (enzyme cofactor) Potential problem? Buffer, containing salt Taq polymerase 3’ 5’ 5’ 3’ 3’ 5’ 3’ 5’ Forward primer Reverse primer Target sequence 6
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H-W and its uses Deviations from theoretical genetic equilibrium are used to show a population is changing over time We can estimate allele frequencies in a population This is ONLY needed if you cannot explicitly determine everyone’s genotypes! ** Genetic equilibrium serves as a null hypothesis and lets us make testable predictions ** If a population is in genetic equilibrium then 7
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Estimating frequencies Phenylketonuria (PKU) Autosomal recessive metabolic disorder Inability to break down phenylalanine leads to toxic accumulation in the body, resulting in developmental abnormalities and mental retardation 8
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Public health officials need to make guidelines for dealing with genetic diseases What we want to know is the likelihood of parents carrying the mutation We can’t geneotype every individual in the world to get an accurate percentage of PKU alleles … What we do know is that the occurrence of individuals with PKU is 1 per 10,000 births What is the genotype of a PKU individual? 9
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This note was uploaded on 04/02/2012 for the course BIO 325L taught by Professor Franks during the Spring '08 term at University of Texas at Austin.

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BIO 325L Lecture 5 - This week in lab 1 Quiz Turn in...

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