BIO 325L Lecture 6 - This Week In Lab Lab activities Arrive...

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This Week In Lab…. Lab activities Arrive on time! Be prepared to load gel immediately Quiz (after gel loaded and running) HW 5 due Fly lab Part 5 (pp.25-28) Evaluate F2 progeny, tabulate individual data Combine section data DNA Typing Lab 2: Part 2 - STR (pp.49-52) Self-DNA – repeat genomic extract if necessary Polymerase chain reaction (PCR) procedure Run gel and record results Mini-practical: mutant fly identification
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What do you need to turn in? This week (2/28-29) Homework assignment 5 Next Monday (3/5 ) - Mid-Term Exam - 6-8PM, UTC 2.112A Next week (3/6-7) HW: Crime lab – match up STRs of “suspect” WA#3 First peer review. Bring four hard copies of your draft to lab. 2
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CHI SQUARE ANALYSIS Last week…..a review
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Frequency Distributions Chi-square analysis : A test for deviations of Observed frequencies from Expected frequencies. The Observed values are those we gather. The Expected values are the frequencies we expected, based on our null hypothesis. To determine if a frequency distribution differs from the expected distribution.… 1. first, calculate chi-squared Chi-squared = sum of (observed-expected) 2 /(expected) 2. second, determine the number of degrees of freedom (i.e., the number of classes of progeny expected minus 1) 3. third, locate the value of X2 value in Table of Critical Values 4 . forth, determine the p-value
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Quiz questions 1.The _________ value is a single number that adds up all the differences between our actual data and the data expected. 2.A bigger difference will give a (bigger) (smaller) Chi-square value. 3.If the actual data and expected data are __________ , the Chi-square value is 0. 4.If there is a greater than 5% (p>0.05) chance that the difference in the observed and expected values is due to random variation, the null hypothesis ( can) (can not) be rejected . 5.If the chance that the difference in the observed and expected values is less than or equal to 5% (p<0.05)the null hypothesis ( can) (can not) be rejected.
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