Quant_Lect_16_Kin - Kinetics Methods Page 1 of 2 Kinetics...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Kinetics Methods of Analysis I. Why use rates instead of waiting for equilibrium? A. Selectivity -- can sense fastest reacting components in the presence of slower reacting interferences. B. Save time. C. Importance of detected substance is its catalytic activity (e.g. enzymes) D. Selection agent only works as a reaction rate transducer (e.g. use of glucose oxidase to sense glucose) II. Common rate expressions. A. First order. A B. d[A]/dt = -k 1 A. [A] = [A] 0 e - kt . [B] = [A] 0 (1 - e - kt ). B. Second order. A + B C or 2A B. d[A]/dt = -k 2 [A][B] or = -k 2 [A] 2 . C. Michaelis Menten. Simplest enzyme model. S + E ES E + P. Steady state approximation gives d[P]/dt = k 2 C E [S]/(K M + [S]). Limiting cases ([S] 0, [S] ∞). Lineweaver-Burke plot: 1/(d[P]/dt) vs. 1/[S]. Slope = K M /(k 2 C E ), Intercept = 1/ (k 2 C E ). D. Even for complex situations, initial rates may be pseudo-first order in analyte III. Common approaches to collecting and using data. A. Initial rate methods.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/02/2012 for the course CHEMISTRY 222 taught by Professor Mcdonald during the Fall '11 term at University of Illinois, Urbana Champaign.

Page1 / 2

Quant_Lect_16_Kin - Kinetics Methods Page 1 of 2 Kinetics...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online