AssignmentTen - min , and assuming a maximum useful...

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Take-Home Assignment #10 Please turn in neatly worked solutions for the following problems before leaving for the Thanksgiving break. Shown on the reverse is a hypothetical chromatogram for a mixture of nine compounds (time is given in minutes). Assume that the retention time for non-retained components is 45 seconds and that the mobile phase’s flow rate is 1.2 mL/min. The column’s length is 15.0 cm. Answer the following: 1. Prepare a table showing the retention time, peak width, retention factor, plate height, and number of theoretical plates for each peak. Report the average number of theoretical plates for the column. 2. What is the volume of mobile phase in the column? Assuming that this is an appropriate estimate for V
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Unformatted text preview: min , and assuming a maximum useful retention time of 30 min, what is this separation’s peak capacity? 3. The two peaks eluting between times of 16 and 18 are poorly resolved. Estimate α and R for the separation of these two components. Suppose you want to improve this resolution to a minimum value of 2.0. The general equation for resolution shows that this can be accomplished by increasing N, α , or k for second of the components. For each of these parameters, determine the minimum value needed to achieve the desired resolution, assuming that the values for the other two parameters remain unchanged. 0.00 5.00 10.00 15.00 20.00 25.00 8.00 10.00 12.00 14.00 16.00 18.00 20.00 Time Signal...
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This note was uploaded on 04/02/2012 for the course CHEMISTRY 222 taught by Professor Mcdonald during the Fall '11 term at University of Illinois, Urbana Champaign.

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AssignmentTen - min , and assuming a maximum useful...

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