BIS 102 MT2 Fall 2011

BIS 102 MT2 Fall 2011 - 1 of 4 BIS 102 Name K93 Fall, 2011...

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Unformatted text preview: 1 of 4 BIS 102 Name K93 Fall, 2011 Last First K. Hilt Second Midterm Score (100): Equations: pH = pKa + log {[b]/[a]} AG = AH - TAS pKa’s of amino acid side chains: D (3.9), E (4.2), H (6.0), C (8.3), Y (10.1), K (10.5), R (12.5) pK, of amino acid a-COOH (2.1); pKfi of amino acid <1t-NH3’r (9.6) pK. of oligopeptide N-terminus (714); pKa of oligopeptide C-terminus (3.6) pKa of NH:+ (9.25) 1. (5 pts.) Antibodies have several disulfide bonds. These tetramers are secreted into the blood. The disulfide bonds would be considered to be part of (circle the letter of the best answer): a) 1° and 2° structure \ b) 2° and 3° structure _5 5% c) 1° and 3° structure 5" :4 (EDP and 4° structure e) 3° and 4" structure 2. (15 pts.) There are three important histidine residues in Hb. Identify them; rank them, and explain what each one does. If they do more than one thing, then list them all. Limit your answers to the lines provided. #1 in importance Name: Pr wimai H W Function(s):\ \Iah MDV¢MGLJ 5‘ Fan” +0 MOW .35: $011? WHEEL a.) Wad; back gar; 3-; FJ" firm «cadre? wz’m H20 #2 in importance Name: QFS‘bJ. [j Function(s): \. l—F" scavenger 2. run-kw H~ buwfl +0 03 3. fierce/s CD 4+» mg oi- M 0449\‘3. #3inimportance Name: Cr+evm\na—Q H {L Q-subuni+$ + l Function(s): | gflvieb wail-e. H 5 gram +1951“: 2. “deg 23g Ana 0 +1, 9,; Cred-{w +I—- ml Wm. 1)qu 2 of 4 BIS 102 Name /75H or 5’ 3. (8 pts.) Predict how the following environmental changes will affect the pKa of a C residue in a protein (circl e correct arrow for each answer): a) a D sidue in the same protein is brought into close proximity ~L@ pKa b) the C side chain shifis from the outside of the protein to a nonpolar 'te inside ~L®pKa c) an R+residue in the same protein, that was close, moves away Jr pKa d) a Kircsidue in the same protein is brought into close proximity. @T pKa 4. (25 pts.) Draw in very carefully the 02 binding curves that you would expect for each of the following situations, using the axes below. Your curves must be clearly labeled with the letter of the situation that you are describing. Assume that Hb has BPG binding to it. Use a dotted line or a dashed line to clearly show how one curve is different from a nearby solid line curve. Your curves must be clear! Situation: a) Hb, pH 7.2 b) Hb, pH 7.6 c) Hb, pH 7.2 with 50% of its sites bound with C02 (1) Hb, pH 7.6 with 75% of its sites bound with CO e) Hb, pH 7.2, but the N-termini of the a-subunits have mutated from a D to a Q <—— 5% \| I! Wvfia/L“ lap 26—- [00 BIS 102 Number of Residues in 3of4 Nam: ‘__K%________________ 5. Amino acid analysis is done on protein “Z”. The following results are obtained: Amino Acid Number of Residues in (25 pts.) a) Calculate the net charge, to the nearest 0.01, of protein “Z” at pH 9.1. Note: there are NH;r ions present in the above table. Show all calculations, either in the table above, in the space below, or on the back of this page. Put your final answer here: net charge = (5 pts.) b) At pH 9.4, would you use order to do ion exchange chromatography. xp am. MAJCAM’QL-is \ ‘11 22 q we am; it. PH; 8Ka+ 4b 4.14; 10,! +105 4. TM nei- dad/IT. :- IN?— “ 2~‘l"l- 7H! — ‘1 + mute. +84 at cation exchangerbr anion exchange resin to bind protein Z, in \ n ' \ '+".’ A ccd't‘m mahaMfiQr \$ " . 4of4 BIS 102 Name g :2} 6. Dr. Anfinsen explored protein folding using RNase A. (2 pts.) a) One of the compounds that he used was urea. Draw its structure here: /c. \ (5 pts.) b) How does urea work in this experiment? Kudns \) M$mplnobic 4 @fo+ein 2.) H' laWuLQ. Eil‘ml‘nam RN‘ALQ Zoo/«J. 3D 5+vuc:\-u.f-¢, 7. (5 pts.) Dr. Levinthal made an important contribution to protein folding. What was that contribution? Explain. “Pate/v." f'olavihé is v10"— (MAW. 8. (5 pts.) Explain how the GroEL/GroES complex works in E. coli. l, His RAM «rel-45M I pol-Ht whopkobfc. mam weasel, \ocnau H bac'Q/ropkolm‘c. Hp i Mi. www. 7.. MMW Qmflin weolal’y a6 H‘ Linda +0 Mqufiwlou‘; limit“) 0",, o‘wamW. 3. Lim‘n7 51 at".th CAM/"3,“ “I” fold-fl oLue. +0 :an r} ATP- . ‘4. Palms“ is released cowl s-lur-ls gelding ajml gang Aown H's enerjv guhnd. ...
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This note was uploaded on 04/03/2012 for the course BIS 102 taught by Professor Hilt during the Spring '08 term at UC Davis.

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BIS 102 MT2 Fall 2011 - 1 of 4 BIS 102 Name K93 Fall, 2011...

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