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BIS 102 MT2 Summer 07

BIS 102 MT2 Summer 07 - 10f2 BIS 102 Name K Summer 2007...

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Unformatted text preview: 10f2 BIS 102 Name K Summer, 2007 Last 5 First K. Hilt Second Midterm Score (100): Equations: pH = pK. + log {[b]/[a]} Ka = 2/(y-x) Kb = x2/ (y-x) pH = (pKall + pKa2)/2 (1<.)(K,,) = 1 x 10'“ Amino acid pK.’s: a-carboxyl group (2.1), a-amino group (9.6) side chains: D (3.9) E (4.2) H (6.0) C (8.3) Y (10.1) K (10.5) R (12.5) Oligopeptide pK.’s: C—terminal carboxyl group (3.6), N-terminal amino group (7.4) Amino acid analysis of protein “Z” gave the following experimental results: Amino Acid Number of ’ ' Number of Residues in Residues in Protein “Z” ' (30 pts.) a) What is the net charge (to the nearest 0.01) of protein “Z” at pH 7‘? Put your answer on the following line: 3 '7 - ‘4 5' . To receive credit, you must show all of your work on the back of this page. (20 pts.) b) Using the above table of information, what “cutting tool” would you choose to cut protein “Z” into fragments suitable for protein sequencing, using the protein sequenator? First identify the cutting tools that we discussed, in class and in the homework, indicating each by name and where each cuts. Then choose only one of the cutting tools and explain why you chose that cutting tool to cut protein “Z”, in order to sequence it using the protein sequenator. Give a good rationale for your choice of the particular cutting tool. Cutting Tools Where It Cuts ~ +l \ It vb “79"“ + K 9‘ l l t CJMjMO'hI-oqsin 4" +1 ammafic a.a. (”I Y) avg- C H 1" it} 1131 vc +1 +1 10" ”G” M +1 +1 “1) Ctdfir +1 +1 H‘b My choice of cutting tool: cuér + I TMyreasonforchoosingthisis: Tab-Q “W (L “.41. MM = 206:, we pundit aJ-(o 4.4.- re$?&u_ piece/.1. 40.» +1“ «(M sewne‘lw. zob 5-_,$‘ 110: +7 W are. 5‘ M—tmon‘cm, use cufir. -3 +24 +20 9”” 9‘“ *' “’3‘: -3 / 7 ; G #12:? a; “”44. W 1 2: {0'3 a. 4- - £52.~r~*":°“ WA “.124. ' w u _‘ “0.013:- 1—5 KPH- 6.4.94 Qfizeuki' (0% a 7: 3234‘ l "1 a ”'6‘“ (-3 = V71; 19-45 - 9:. ’7‘” "’ b . b: .5— : are-:— 2035- —o.o~¢77 Q mm =- +5 w = aw 20f2 BIS 102 Name 9:2 2. (35 pts.) Using the axes provided below, draw in very carefully the expected 02 binding curves for each of the following situations. Label each curve with the letter that describes it, i.e. “a”, “b”, etc. Then give explanations for just parts “i” and “g”. c_ % 02 bound +3 a) Hb, pH 7.2, with BPG *3. b) Mb, pH 72 4.... only) ”we, WA" ‘5: “DWVDUL. + 3 c) Hb, pH 7.6, with BPG +3 d) Hb, pH 7.6, stripped of all BPG *— s+m mam, gtamutm 4/21 t 3 e) Hb, pH 7.6, with BPG, and 75% of the binding sites bound to CO 2 1.3 t) Hb, pH 7.2, with BPG, but the N-terminal amino acid residue of each a-subunit has been changed g to an alanine \ J, a g) Hb, p572, with BPG, but all N-terminal amino groups had be? chemically changed to amides cwwe/ is somem be¥wwm°aflm AM .Ws M6 sifivWD‘fl-a—Q” Explain your angers for parts “i” and “g” above: *3 Reasonforpart“f’: C’WB‘IB' ‘RM. R-1(°\T 3.0“— M W m d»- “HZ afoue’ +é% which. Rs W Mar W} bim&4,+3 Reasonforpart“g”: AMIM cannot? btu9~ “*fz’flm. H". Qofl-‘ww 01—h BO‘W' +46 g M “M beam. «‘er lav-1' no‘i‘ difimfaH-l- " M 5H“ $909» ‘3' Rae. C- Wm. . NS‘Hadnfl/L a-L +6.1 fi- Swiwwui‘i'fi +3 3. (15 pts.) Would the pKa of an N-terminal amino group of a protein go up or down if the following occurred: H" a) a K‘rside chain that was close, moved away? T “’ a b) an E'side chain that was far away, moved into close proximity? 1 " Z c) the N-terminal moved out of a cluster of W side chains, into a cluster of S residues? 1 4' 7' Give your reasoning for your answer in part “c” above: v.) . °\Q’;“ In clay ‘ M A.“ A Thu; 5 an «\on . a “one ehvhron I Ha. M- M- N ,. iju-Q wow. no 4'? Tbs-.4. is accawflisktfl by min}a 4,0[1’6 Mom} 4-. a- c,\w5W 6‘. swim 7 Wick are (fob/T: raw-Ml in 3M Arne «18°54'9— and.“ ...
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