Key HW _2 W 2012

Key HW _2 W 2012 - 1 of 3 Biological Sciences 102 Winter,...

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1 of 3 Biological Sciences 102 Answer Key Winter, 2012 K. Hilt Homework #2 1. Amino acid analysis is done on protein “Z”. The following results are obtained: Amino Acid Number of Residues in Protein “Z” Amino Acid Number of Residues in Protein “Z” A 13 B 13 G 12 W 6 P 5 H 12 Z 24 C 4 K 8 V 24 R 12 S 12 L 23 T 14 Y 12 F 13 a) Calculate the net charge, to the nearest 0.01, of protein “Z” at pH 7. Use the pK a ’s given in homework #1. “–16.4” Use the pK a ’s of 7.4 and 3.6 for the N- and C-termini. The charge coming from lysines in protein Z would be +8. Whenever the pH is closer than two pH units to a pK a , you need to use the Henderson-Hasselbalch equation. b) What is the molecular weight of protein “Z”? Use the value of 110 Daltons as an average molecular weight of an amino acid residue. 22,770 c) At pH 7, would you use a cation exchanger or anion exchange resin to bind protein Z, in order to do ion exchange chromatography? anion exchanger d) Does protein “Z” have quaternary structure? How would you experimentally confirm this? We do not know, from the information given above. What would you propose to find out?
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This note was uploaded on 04/03/2012 for the course BIS 102 taught by Professor Hilt during the Spring '08 term at UC Davis.

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Key HW _2 W 2012 - 1 of 3 Biological Sciences 102 Winter,...

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