Bio Lab Ana #3 - Group Members: _Date: _ LI _ Unique _ Day:...

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Group Members: _______________________________________________________________Date: ___________ LI ______________________ Unique __________ Day: __________ Room: _______ Lab Start Time ________ Exercise 3 Analysis (1) Answer the following questions concerning the use of immersion oil with brightfield microscopy. (a.)What is the purpose of immersion oil? Immersion oil is used to minimize the reflection of light, which leaves the object at large angles. This is due to the fact that the oil has a refractive index closer to that of glass than the air. The immersion oil helps improve the quality of the image with high-magnification objective lenses. (b.) For the microscopes used in this exercise, which objectives require the use of immersion oil? How can you tell ? The 100X objective must be used with the immersion oil. The objectives are labeled OIL, OEL, or HI on the casing of the objective if they are designated for use with immersion oil. (c.) If an objective is designated for use with immersion oil, must it be used? Can immersion oil be used with other non-oil-rated objectives? The immersion oil must be used if an objective is intended for that purpose. The immersion oil cannot be used with other non-oil-rated objectives because it would decrease the quality images of the specimen. (d.)Describe the proper way to place immersion oil on a slide and use it with your oil immersion objective. The steps to properly place immersion oil on a slide: Put specimen on sharp focus and view it under 40X objective Rotate the nosepiece on the position halfway between 40X and 100X objective Place a drop of immersion oil on the small light spot on specimen Then carefully slide 100X objective into the oil alignment without striking the slide. Make sure the very tip of the lens contacts the oil only. (e.)Why must the focal plane never be adjusted when adding immersion oil? The focal plane should never be adjusted when adding immersion oil because the objective could hit the oil improperly on the specimen, damaging either the specimen or the objective. It could also smear the oil all over the specimen other than the small light spot only. The focus should be already adjusted before adding the oil. (f.) After immersion oil has been used, what must you do before you can examine a slide again at lower magnification? After the 100X objective with immersion oil have been used, we lower the stage and immediately clean the 100X objective lens and the specimen slide with special lens paper and solution of soap (1%SDS). Then the lower magnification objective can be rotate into the alignment for viewing. (2) Based on your observations in Section 3.1, compare red and white blood cells. (a.)
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Bio Lab Ana #3 - Group Members: _Date: _ LI _ Unique _ Day:...

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