Microbial Metabolism

Microbial Metabolism - METABOLISM: Source of Energy...

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METABOLISM: Source of Energy Phototrophs vs. Chemotrophs Sunlight Carbohydrates Lipids Proteins Inorganic compounds
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METABOLISM: Mechanisms Three mechanisms to extract electrons and Energy from the substrate: I. Aerobic Respiration II. Anaerobic Respiration III. Fermentation
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I . Glucose Metabolism by Aerobic Respiration C 6 H 12 O 6 + 6O 2 6CO 2 + 6H 2 O+ 38ATP 1. Glycolysis (EMP/Entner-D/PPP) 2. Kreb’s Cycle/TCA 3. Electron Transport Chain
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Figure 5.11 Overview of Respiration and Fermentation
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AerobicRespiration : 1.Glycolysis/EMP 1) Embden Meyerhof Parnas Pathway 2) Does not require oxygen, but can occur in presence or absence of oxygen. 3) Used to begin the break down of Glucose. 4) Involves incomplete oxidation of Glucose to Pyruvic acid.
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Yield in Glycolysis 2 Pyruvic acid 2 ATP 2 NADH ATP produced by substrate level phosphorylation
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Oxidation of Glucose Removal of electrons Electron Carriers Release of Free Energy ATP Extract as many electrons as possible from the substrate and then use that energy to form ATP. (Why ATP?)
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1. Currency of Energy 2. Structure of ATP
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• ATP is generated by the phosphorylation of ADP with inorganic phosphate. This is an energy requiring (endergonic) process. a) Substrate level phosphorylation: Synthesis of ATP using the energy released in an exergonic reaction b) Oxidative phosphorylation: Synthesis of ATP using the energy of an electron transport chain, ETC. (Proton motive force) c) Photophosphorylation
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Microbial Metabolism - METABOLISM: Source of Energy...

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