Unit 3Transcription Translation Gene Expression Mutation Spring 09 for BB

Unit 3Transcription Translation Gene Expression Mutation Spring 09 for BB

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Unformatted text preview: Transcription The Expression of Genes in the Cell Gene Expression At the molecular level, a gene is an organized unit of DNA sequences that enables a segment of DNA to be transcribed into RNA, and ultimately results in the formation of a functional product The gene may be a structural gene that codes for a final polypeptide product, or The gene may code for tRNA or rRNA as the final product Gene Organization Sequences organized in a way that allows the DNA to be transcribed to RNA Gene Expression in Prokaryotic Cells The Operon Jacob & Monod lac operon inducible The lac Operon The trp Operon The trp Operon Positive Gene Regulation Transcription Requires four ribonucleotide triphosphates to make a complementary copy of the DNA Sequence of bases in RNA is determined by the base sequence of the DNA Although the DNA molecule is double-stranded, at the time any particular region is transcribed, only one strands serves as a template RNA polymerase of E. coli is one of the largest enzymes known and can be seen by electron microscopy RNA polymerases are able to initiate chain growth directly without a primer Transcription Occurs in three stages Initiation: Recognition step Sigma factor binds to RNA polymerase which synthesizes RNA Sigma factor also recognizes the base sequence of a promoter and binds there Elongation: synthesis of the RNA transcript Sigma factor is released and RNA polymerase moves along DNA strand RNA is synthesized in the 5-3 direction Termination: RNA polymerase reaches a termination sequence Stages of Transcription Multiple GeneTranscription Eukaryotic Transcription Spliceosome RNA Processing Translation What is a protein? A protein is a polymer composed of monomers called amino acids General Structure of an Amino Acid Amino Acids Amino Acids...
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This note was uploaded on 04/05/2012 for the course BIO 231 taught by Professor Nette during the Spring '11 term at Cuyamaca College.

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Unit 3Transcription Translation Gene Expression Mutation Spring 09 for BB

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