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Unformatted text preview: L of pMCT118. The bottom of the test tube was tapped until the solution transformed into a pink color. A new test tube was used to mix 2.5 L of the cell sample in the PCR test tube. The test tube was spun in a microcentrifuge for one second. The test tubes were cooled on ice before loaded into the thermal cycler. PCR was achieved in the following conditions: 30 cycles of 94C for 30 seconds, 65C for 30 seconds, and 72C for 30 seconds. The PCR product was stored at 4C....
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This note was uploaded on 04/05/2012 for the course BIO 141 taught by Professor Dr.cafferty during the Spring '11 term at Emory.
- Spring '11