BIO 317 - Lec 5

BIO 317 - Lec 5 - BIO 317: Lecture 5 Prof. William Collins...

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1 Prof. William Collins OfFce: 534 Life Sciences Building OfFce Hours: Mondays, 4:00 – 5:00 PM or by appointment Recommended Reading: Matthews, Chapter 7 BIO 317: Lecture 5 Patch Clamp Measurements for a Single Voltage-gated Na + Channel
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2 Gating in Single Channels From: Ion Channels of Excitable Membranes by B. Hille Molecular Structure of Voltage-gated Na + Channel The Na + channel is one large protein • 2,000 amino acids • 4 domains of similar make-up, I-IV • Each domain has 6 transmembrane segments, S1-S6 • S4 in each segment is highly positively charged (arginine & lysine residues) (voltage sensor) • External loop connecting S5 & S6 in each domain lines pore • Cytosolic loop connecting domains III and IV is involved in inactivation gating
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3 Voltage-gating of Na + Channel “The axon doesn’t think. It only ax.” - George Bishop, 1965 • Axons are highly specialized for conduction • APs are brief and can follow at high frequencies • Only AP timing is important (time and frequency) not AP amplitude or duration • 2 major voltage-gated channel types – Na + , K +
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BIO 317 - Lec 5 - BIO 317: Lecture 5 Prof. William Collins...

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