Midterm 2_LongKEY

Midterm 2_LongKEY - BIS101-002/Draper Midterm II KEY Form A...

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1 BIS101-002/Draper Midterm II KEY Form A 1. DNA Pol I A. 5’ 3’ exonuclease , which it uses to remove RNA primer 2. DNA Pol III E. None of the above 3. Primase C. 5’ 3’ ribonuc. synthesis to make RNA primer for lagging stand synthesis 4. Ligase B. ATP – dependent 3’-5’ joining 5. Helicase E. None of the above . Helicase unwinds DNA ahead of the replication fork and Topoisomerase/Gyrase remove supercoils that Helicase creates 6. B. 3’ 5’ exonuclease is used to profread and remove misincorporated bases 7. C. Minimal media + Bio and Str. You do not add Gal (i.e. galactose) because this would select for Gal+ colonies (only gal+ can grow on galactos). 8. B. bio-gal-arg : no colonies for arg+ gal- bio- were obtained, so this must require quadruple cross over. This indicates gal is in the middle. This recombinant will look like this (follow the red to get arg+ gal- bio-): ------ ---arg+--- ----gal+----- ---bio - --- ------ 1 / 2 \ 3 / 4 \ ----- ----arg - ---- ---gal - ----- ---bio+--- ------ 9. B. 2 mu : Add up recomb. events in interval 3 = arg+ gal+bio+ = 8/400 = 2% 10. What is the cotransduction frequency of pur and nad? = A. 26% : count all pur+ nad+ colonies and divide by total (51). pur + nad+ pdx+ = 3 pur + nad+ pdx- = 10 = 13/51 = ~26% 11. What is the cotransuction frequency of pur and pdx? C. 46% : count all pur+ pdx- colonies and divide by total (51). pur + nad+ pdx- = 10 pur + nad- pdx- = 13 = 23/51 = ~46% 12. Which of the unselected loci is closest to pur? B. pdx : co-transduction frequency increases as genes get closer.
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2 13. What is the gene order? There are two assumptions that you could have made when approaching this
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This note was uploaded on 04/05/2012 for the course CHE 52299 taught by Professor Nambiar during the Spring '08 term at UC Davis.

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Midterm 2_LongKEY - BIS101-002/Draper Midterm II KEY Form A...

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