Homework07

Homework07 - I P O Z Y 2 Is P O Z Y I P O Z Y 3 Is P Oc Z Y...

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BIS101/Draper Week#7 suggested problems and homework Problems suggest for the whole class – these are not to be turned in, but are for practice/study aid. 8 th edition Chapter 10: 2, 3, 4, 5, 6, 21, 24 9 th edition Chapter 10: 2, 3, 4, 5, 6, 9,11 101D assignment #7 is listed on the next page (due the week of May 16). These questions are taken directly from past test. The key to the 101D assignment will be posted on SmartSite May 20 after 1:00pm. CORRECTED Version (5/19/2011)
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1). In the lac operon, the repressor gene is (I), the promoter is (P), the operator is (O) and the structural genes are ß-galatosidase (Z) and permease (Y). (23 pts) a). For each of the following examples of the lac operon partial diploids, predict whether the expression of ß-gal and permease are inducible , constitutive , or absent . (12 pts) GENOTYPE ß-gal Permease 1) I+ P+ Oc Z+ Y-
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Unformatted text preview: / I+ P+ O+ Z- Y+ 2) Is P+ O+ Z- Y- / I- P+ O+ Z+ Y+ 3) Is P+ Oc Z- Y+ / I- P+ O+ Z+ Y- 4) Is P+ O+ Z+ Y- / I- P- Oc Z- Y+ 5) I- P+ O+ Z+ Y- / I+ P+ Oc Z- Y+ 6) I- P+ Oc Z+ Y- / I+ P+ O+ Z- Y+ b). Considering the elements I, P and O, which of these function in cis and which function in trans ? (3 pts) cis functioning:________________ trans fuctioning:_______________ c). The graph below depicts the results of an experiment performed by Pardee, Jacob and Manod. In this experiment, they measured the relative amounts of ß-galatosidase protein synthesis in an lacI- lacZ-E. coli mutant after introduction of the lacI+ lacZ+ genes via transformation at time = 0. Using your knowledge of the lac operon, explain why, in the absence of inducer, ß-galactosidase protein levels initially accumulate but then decline. (8 pts)...
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Homework07 - I P O Z Y 2 Is P O Z Y I P O Z Y 3 Is P Oc Z Y...

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