Homework05_KEY

Homework05_KEY - BIS101/Draper Homework05_KEY 3. (10 pts)...

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BIS101/Draper Homework05_KEY 3. (10 pts) Below are 50 base pairs of a sequence from the zebrafish dmc1 gene, which encodes a protein that is required for meiotic recombination. 5’-GTGCTTCCTTACAAGAAACCGGGGAAGTACATCCGTCTCGCATGTGACCG-3’ a. (4 pts) Design two 10-mer oligonucleotide primers that could be used in a polymerase chain reaction to amplify the above 50 base pair fragment of the dmc1 gene. Forward primer: 5’- G T G C T T C C T T -3’ Reverse primer: 5’- C G G T C A C A T G -3’ b. (2 pts) Which of the above primers will have the highest Tm? Explain your reasoning. The reverse primer will have the highest Tm because it has 6 G/C bases, while the forward primer has only 5 G/C bases. c. (4 pts) If the sequence above represents the “non-template strand,” write out the first 10 bases of the mRNA sequence that could be transcribed from this region of the dmc1 gene. mRNA 5’- G U G C U U C C U U -3’
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2. (14 pts) Four different rII - deletion strains of phage T4 were crossed pair-wise in
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Homework05_KEY - BIS101/Draper Homework05_KEY 3. (10 pts)...

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