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Lecture08_26Apr11

Lecture08_26Apr11 - 2 Must allow for faithful replication...

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Bacterial Genetics II BIS101 April 26, 2011
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Growth in media containing: Are these cells Mating glucose/str glucose/met/srp lactose/str lactose/met/str Donors? A X D yes yes no no yes B X D no yes no no yes C X D no yes no yes no Does it form a conjugation Growth in minimal media containing: Strain tube? glucose lactose glucose/met glucose/met/str lactose/met/str A yes yes yes yes no no B yes yes yes yes no no C yes yes yes yes no no D no no no yes yes no From “Transduction Handout” /str
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Transformation
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Bacteriaphage
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Bacteriaphage
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Bacteriaphage QuickTimeª and a decompressor are needed to see this picture.
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Bacteriaphage
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Bacteria phage T4 1.7 X10 5 bp genome encodes about 300 gene products (E. coli = 4.6 X10 6 bp)
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Generalized Transduction
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Co-transduction
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DNA
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Qualities necessary for the structural features of the genetic material: 1. Must have informational content (to encode for proteins)
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Unformatted text preview: 2. Must allow for faithful replication during each cell division 3. Must have physical and chemical stability 3. Must be able to change (e.g. by mutation-more in Chapter 14 ). DNA is the Genetic Material Griffith, 1928 DNA is the Genetic Material Avery, MacLeod and McCarty, 1944 DNA is the Genetic Material Hershey-Chase, 1952 Rosalind Franklin Franklin’s X-ray diffraction pattern of DNA James Watson and Francis Crick 1962 Nobel Prize in Physiology or Medicine The Structure of DNA Qualities necessary for the structural features of the genetic material: 1. Must have informational content (to encode for proteins) 2. Must allow for faithful replication during each cell division 3. Must have physical and chemical stability 3. Must be able to change (e.g. by mutation-more in Chapter 14 )....
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