Lecture14-S11 - BIS 101 19 May 11 Regulation of Gene...

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BIS 101 19 May 11 Regulation of Gene Expression Part 2: Eukaryotes
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Handout trp mRNA leader sequence contains an attenuator region and two Trp codons Met Trp Trp
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Handout Trp Trp Mechanism of Attenuation: trp Operon
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Leader Peptides of Amino Acid biosynthesis Operons
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Regulated Gene Expression gene 1 gene 1 Ear Brain Kindey A zebrafish embryo (15 hour post-fertilization)
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Figure 10-22
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Figure 10-23
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Ziwi promoter drives heterologous gene expression in all germ cells EGFP 4.8 kb Ziwi 5’ Tol2 Tol2 ziwi in situ Tg[ziwi:EGFP] David Leu (CDB)
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Ziwi promoter drives heterologous gene expression in all germ cells EGFP 4.8 kb Ziwi 5’ Tol2 Tol2 ziwi in situ Tg[ziwi:EGFP] David Leu (CDB)
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4.1 kb 3.08 kb 2.26 kb 1.3 kb EGFP EGFP EGFP EGFP pBD286 pBD287 pBD288 pBD289 pBD287 pBD289 MscI Sal I Ava II Mapping ziwi enhancers GFP+ GC (16 dpf) 7/15(1) 72/278(3) 8/18(1) 4/30(1) Exp. Level Strong Strong Strong Weak 0.7
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Model Eukaryote: Budding Yeast Saccharomyces cerivisiae
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The Gal pathway in Yeast
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Gal4 is a Sequence-Specific DNA Biding protein that Positively Regulates Transcription UAS = Upstream Activating Sequence
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+ + + Major Grove Minor Grove Minor Grove Major Grove DNA Binding Proteins
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Gal80 and Gal3 Regulates Gal4 Activity
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Txn Activators Recruit Txn Machinery Coactivator
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Transcriptional Activator Proteins are Modular
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X-gal
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