Lab 9methods - Introduction Respiration is a common...

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Introduction Respiration is a common occurrence in every cell of living organisms and involves the consumption of oxygen. It is a catabolic reaction that involves the breakdown of glucose and other nutrients. (Robinson, 2010) There are different stages of respiration and they all involve enzymes. (Russel, 2010) Respiration in plants begins when the seed absorbs the water being that was provided. (Jinapang, 2010) The oxygen produced during respiration is used to convert stored nutrients in the seed into energy that the seed uses to sprout. Throughout a plant’s development the respiratory rate will change. (Smillie, 2010) Temperature, moisture, light exposure, carbon dioxide and oxygen levels affect the rate of respiration. (Headford, 2010)In this experiment the different stages of development were being compared, so all of these variables were kept at a constant. Mung bean sprouts were used to analyze the comparison of respiratory rates at different stages of plant growth. Mung bean sprouts are generally grown using water and very little sunlight, most of the time they are kept in the dark. (Tawfik, 2008) In general, the respiration rate declines gradually with time after the plant reaches a certain point. This decline may be attributed to the effect of the law of mass action or to a general aging effect. (Smillie, 2010) Due to this fact it was hypothesized that there would be a difference in respiratory rate in mung bean sprouts at different stages of growth. There was no light involved in this experiment and the mass of the organisms was kept similar. There were two test tubes with organisms of different age, and a single test tube which was used as a control that had no organism inside. The rate of respiration is the measure of oxygen being consumed by the organism over a certain period of
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time. The rate of oxygen consumption was calculated using the unit mL of oxygen consumed per hour, divided by the weight of the organism. Materials and Method
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Materials: 3 test tubes Test tube rack Stopwatch Mung bean sprouts Pipette Syringe Gloves Kleenex Water Scale 3 soda limes Procedure: This experiment was performed by Hamasha and I. Firstly, a measuring scale was used to measure approximately 2.0 g of mung bean
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Lab 9methods - Introduction Respiration is a common...

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