plant_pigment_new_exp -...

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Separation and Identification of Plant Pigments Dr. Gergens  - SD Mesa College PURPOSE In this experiment, the photosynthetic pigments common to all flowering plants will be extracted by liquid-liquid extraction.  The four main pigment components of plant leaves are chlorophyll a, chlorophyll b, carotene, and xanthophyll.  The solvents  needed for the extraction and separation will be petroleum ether, diethyl ether, methanol, and water.  Finally, these pigments  will be analyzed by measuring the absorption spectra of the plant pigments. BACKGROUND The four main pigment components of plant leaves are chlorophyll a, chlorophyll b, carotene, and xanthophyll.  While most  plant leaves appear green to our eyes, several different photosynthetic pigments of various colors are usually present in the  chloroplast of green plants.   The colors of these pigments are due to the numerous  double bonds in their structure.  Chlorophyll a and b provide the green color of leaves, which absorb most of the light energy needed for photosynthesis to   occur.  The greatest absorption of light by chlorophyll is in the red, blue and violet wavelengths.  Associated with these green  pigments are several other pigments known as carotenoids.   These are commonly yellow to red in color, and are also  involved in harvesting light energy for photosynthesis. 1
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RESULTS/DISCUSSION (include the following in your formal conclusion) 1. Include graphs of the four spectra for chlorophylls a and b, carotene and xanthophyll 2. Include structures for each pigment.  Paste the structure of each compound into the appropriate cell.  If necessary do   the following <Insert>, <Paste Special>, <Picture> and turn off [float over text] 3. Tabulate the wavelength at absorbency maxima for each of the four pigments. 4. State the color(s) of visible light each pigment absorbs. 5. What color does each pigment appear to our eyes?   In other words, state the color(s) of visible light each pigment   transmits. Pigment Chlorophyll a Carotene Chlorophyll b Xanthophyll Structure (import from SigmaAldrich web site) (import from SigmaAldrich web site) (import from the Galactic web site) (import from the SDBS, Japanese web site) Wavelength(s) of absorbency  maxima The color of visible light the pigment absorbs: To our eyes the pigment appears (give color) 6. Structurally what are the major differences between chlorophyll a and b? 7.
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This note was uploaded on 04/06/2012 for the course BIOL BIOL 1010 taught by Professor Comptonw during the Winter '09 term at York University.

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plant_pigment_new_exp -...

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